Automated subsequent era sequencing platform can precisely display 1000’s for COVID-19

Javier Hernandez is pictured within the Wrana lab on the Lunenfeld-Tanenbaum Analysis Institute in Toronto, Canada, on this undated file picture. Credit score: Sinai Well being

A robotics platform designed by Toronto researchers to display 1000’s of COVID-19 samples directly has the potential to revolutionize how labs observe the unfold of viruses and different pathogens, in keeping with new findings.

The examine, out Wednesday in Nature Communications, discovered that the next-generation, ultra-high-throughput sequencing platform, known as C19-SPAR-Seq, designed by researchers from the Lunenfeld-Tanenbaum Analysis Institute (LTRI) at Sinai Well being, has a sensitivity charge higher than 95 % in optimistic circumstances throughout peak onset.

“Figuring out optimistic samples shortly and precisely is essential in beating this pandemic,” stated Dr. Jeff Wrana, senior investigator on the LTRI and professor within the Division of Molecular Genetics on the College of Toronto. “With new and probably harmful variants now circulating, this can be a platform that’s scalable, automated and able to analyzing 1000’s of COVID-19 affected person samples in a single instrument run.”

Wrana and fellow LTRI senior investigator Dr. Laurence Pelletier, in collaboration with College of Toronto professor Dr. Ben Blencowe, credit score a robust crew of keen trainees who shifted from different areas of analysis to assist develop and validate the platform, permitting for the crew to go from idea to revealed paper in below 12 months.

“The co-operation of the Mount Sinai Hospital medical diagnostic lab was the opposite key ingredient to our success,” stated Pelletier. “Thus far the shared microbiology lab, headed by Dr. Tony Mazzulli, has offered entry to 1000’s of samples.”

In late 2020, the crew pivoted once more to make use of the robotics platform to display 1000’s of optimistic samples for variants by quickly sequencing fingerprint areas of the viral genome to search for key mutations.

“It has been an absolute pleasure to work with Dr. Jeff Wrana and his crew on the LTRI,” stated Dr. Mazzulli, microbiologist-in-chief for Sinai Well being and College Well being Community (UHN). “His novel SPAR-Seq System is cutting-edge expertise and his crew’s skill to sequence COVID-19 samples in actual time has great potential for impacting our understanding of the epidemiology and unfold of novel mutants within the province.”

The platform can also be cost-effective. The examine notes it solely prices about $8 USD per take a look at when operating 1000’s of samples directly, as the price per pattern decreases as a consequence of economies of scale.

“It is extraordinarily dependable and readily adaptable,” stated Javier Hernandez, a junior researcher within the Wrana lab who co-led the examine with Drs. Marie-Ming Aynaud and Seda Barutcu. “The turnaround is roughly 24 hours. It is quite simple as we have automated virtually each step within the course of. For me, it has been a really thrilling factor to see my work make a distinction.”

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Extra info:
Nature Communications (2021). DOI: 10.1038/s41467-021-21653-y

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Lunenfeld-Tanenbaum Analysis Institute

Automated subsequent era sequencing platform can precisely display 1000’s for COVID-19 (2021, March 3)
retrieved 3 March 2021

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